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Stat1 (phospho Ser727) Polyclonal Antibody

Stat1 (phospho Ser727) Polyclonal Antibody

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Specification

Product name Stat1 (phospho Ser727) Polyclonal Antibody
Immunogen Synthesized peptide derived from human Stat1 around the phosphorylation site of S727
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative STAT1; Signal transducer and activator of transcription 1-alpha/beta; Transcription factor ISGF-3 components p91/p84

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The protein encoded by STAT1 (signal transducer and activator of transcription 1) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described.
Gene ID 6772
Alternative STAT1; Signal transducer and activator of transcription 1-alpha/beta; Transcription factor ISGF-3 components p91/p84
Others Phospho-Stat1 (S727) Polyclonal Antibody detects endogenous levels of Stat1 protein only when phosphorylated at S727.
Accession P42224
Observed Band(KD) 87

Image & description

Fig. Western Blot analysis of K562 293 PC-3 HepG2-UV cells using Phospho-Stat1 (S727) Polyclonal Antibody diluted at 1:1000.

Fig. Western Blot analysis of K562 293 PC-3 HepG2-UV cells using Phospho-Stat1 (S727) Polyclonal Antibody diluted at 1:1000.

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