| Product name | SRp20 Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the C-terminal region of human SRp20 at AA range: 80-160 |
| Host | Rabbit |
| Reactivity | Human,Mouse |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:5000;Not yet tested in other applications. |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | SRSF3; SFRS3; SRP20; Serine/arginine-rich splicing factor 3; Pre-mRNA-splicing factor SRP20; Splicing factor; arginine/serine-rich 3 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 25kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Serine/arginine-rich splicing factor 3 encoded by SRSF3 is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors, which constitute part of the spliceosome. Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. Two transcript variants, one protein-coding and the other non-coding, have been found for this gene. |
| Gene ID | 6428 |
| Alternative | SRSF3; SFRS3; SRP20; Serine/arginine-rich splicing factor 3; Pre-mRNA-splicing factor SRP20; Splicing factor; arginine/serine-rich 3 |
| Others | SRp20 Polyclonal Antibody detects endogenous levels of SRp20 protein. |
| Accession | P84103 |
| Observed Band(KD) | 25 |
Fig.1. Western Blot analysis of various cells using SRp20 Polyclonal Antibody diluted at 1:2000.
Fig.2. Western Blot analysis of Jurkat cells using SRp20 Polyclonal Antibody diluted at 1:2000.
Fig.3. Western blot analysis of KB lysis using SRp20 antibody. Antibody was diluted at 1:2000.
Fig.4. Western blot analysis of 293T lysate using SRp20 antibody. Antibody was diluted at 1:2000.
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