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Crk II (phospho Tyr221) Polyclonal Antibody

Crk II (phospho Tyr221) Polyclonal Antibody

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Specification

Product name Crk II (phospho Tyr221) Polyclonal Antibody
Immunogen Synthesized peptide derived from human Crk II around the phosphorylation site of Y221
Host Rabbit
Reactivity Human, Monkey, Mouse, Rat
Applications ELISA, IF, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IF (1:200-1:1000), ELISA (1:40000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative CRK; Adapter molecule crk; Proto-oncogene c-Crk; p38

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background CRK encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of CRK (CRK proto-oncogene, adaptor protein) has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described.
Gene ID 1398
Alternative CRK; Adapter molecule crk; Proto-oncogene c-Crk; p38
Others Phospho-Crk II (Y221) Polyclonal Antibody detects endogenous levels of Crk II protein only when phosphorylated at Y221.
Accession P46108
Observed Band(KD) 40

Image & description

Fig. Western Blot analysis of various cells using Phospho-Crk II (Y221) Polyclonal Antibody.

Fig. Western Blot analysis of various cells using Phospho-Crk II (Y221) Polyclonal Antibody.

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