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SNAI 1 Polyclonal Antibody

SNAI 1 Polyclonal Antibody

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Specification

Product name SNAI 1 Polyclonal Antibody
Immunogen Synthesized peptide derived from human SNAI 1 around the non-phosphorylation site of S246
Host Rabbit
Reactivity Human, Monkey, Mouse
Applications ELISA, IF, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IF (1:200-1:1000), IHC-P (1:100-1:300), ELISA (1:5000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative SNAI1; SNAH; Zinc finger protein SNAI1; Protein snail homolog 1; Protein sna

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 29 KD
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The Drosophila embryonic protein snail is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The zinc finger protein SNAI1 encoded by SNAI1 is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2.
Gene ID 6615
Alternative SNAI1; SNAH; Zinc finger protein SNAI1; Protein snail homolog 1; Protein sna
Others SNAI 1 Polyclonal Antibody detects endogenous levels of SNAI 1 protein.
Accession O95863

Image & description

Fig.1. Western Blot analysis of various cells using SNAI 1 Polyclonal Antibody diluted at 1:1000.

Fig.1. Western Blot analysis of various cells using SNAI 1 Polyclonal Antibody diluted at 1:1000.

Fig.2. Immunofluorescence analysis of rat heart tissue. 1, SNAI 1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.2. Immunofluorescence analysis of rat heart tissue. 1, SNAI 1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

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