| Product name | Smad3 Mouse Monoclonal Antibody (4C9) |
| Immunogen | Synthetic Peptide of Smad3 at AA range of 350-430 |
| Host | Mouse |
| Reactivity | Human,Rat,Mouse |
| Applications | WB,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:1000-2000;IF 1:50-200 |
| Clonality | Monoclonal |
| Preparation method | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Alternative | SMAD3 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 52kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | The protein encoded by SMAD3 (SMAD family member 3) belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene ‘mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. |
| Gene ID | 4088 |
| Alternative | SMAD3 |
| Others | Smad3 protein detects endogenous levels of SMAD3. |
| Accession | P84022 |
Fig.1. Immunohistochemical analysis of paraffin-embedded Human Liver Carcinoma Tissue using Smad3 Mouse mAb diluted at 1:200.
Fig.2. Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using Smad3 Mouse mAb diluted at 1:200.
Fig.3. Western blot analysis of 1) Mouse Brain Tissue Lysate, 2) Rat Brain Tissue Lysate using Smad3 Mouse mAb diluted at 1:2000.
You must be logged in to post a review.
Reviews
There are no reviews yet.