| Product name | Human VEGF165 protein |
| Sequence | Amino acid sequence derived from human VEGF165 isoform (P15692-4) (Met1-Arg191) was expressed. Human and Cynomolgus VEGF165 sequences are identical. |
| Activity | Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 4-16 ng/mL. The ED50 for this effect is typically 4-16 ng/mL. |
| Protein length | The recombinant human VEGF165 consists of 165 amino acids and predicts a molecular mass of 19.2 KDa. It migrates as an approximately 20 and 22 KDa band in SDS-PAGE under reducing conditions. |
| Preparation method | HEK293 Cells |
| Purity | > 95 % as determined by SDS-PAGE |
| Alternative | MVCD1; VEGF; VEGF165; VPF; Vascular endothelial growth factor A; VEGFA |
| Formulation | Lyophilized from sterile 100 mM Glycine, 10 mM NaCl, pH 7.0. |
| Molecular weight | 19.2 kDa |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human VEGF165 protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Storage instructions | Lyophilized Human VEGF165 proteinshould be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.62% HSA or BSA). Please prevent freeze-thaw cycles. |
| Shipping | The product is shipped at ambient temperature. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF) and VEGF-A, is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the platelet-derived growth factor (PDGF)/vascular endothelial growth factor (VEGF) family and often exists as a disulfide-linked homodimer. VEGF-A protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, inhibiting apoptosis and tumor growth. VEGF-A protein is also a vasodilator that increases microvascular permeability, thus it was originally referred to as vascular permeability factor. |
| Gene ID | 7422 |
| Alternative | MVCD1; VEGF; VEGF165; VPF; Vascular endothelial growth factor A; VEGFA |
| Others | P15692-4 |
| Accession | P15692 |
Fig.SDS-PAGE analysis of Human VEGF165 protein.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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