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Smad2/3 (phospho Thr8) Polyclonal Antibody

Smad2/3 (phospho Thr8) Polyclonal Antibody

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Specification

Product name Smad2/3 (phospho Thr8) Polyclonal Antibody
Immunogen Synthesized peptide derived from human Smad2/3 around the phosphorylation site of T8
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:10000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative SMAD2; MADH2; MADR2; Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; JV18-1; Mad-related protein 2; hMAD-2; SMAD family member 2; SMAD 2; Smad2; hSMAD2; SMAD3; MADH3; Mothers against decapentaplegic

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 48 KD
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background SMAD family member 2 encoded by SMAD2 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for SMAD2.
Gene ID 4087/4088
Alternative SMAD2; MADH2; MADR2; Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; JV18-1; Mad-related protein 2; hMAD-2; SMAD family member 2; SMAD 2; Smad2; hSMAD2; SMAD3; MADH3; Mothers against decapentaplegic
Others Phospho-Smad2/3 (T8) Polyclonal Antibody detects endogenous levels of Smad2/3 protein only when phosphorylated at T8.
Accession Q15796/P84022

Image & description

Fig.1. Western Blot analysis of various cells using Phospho-Smad2/3 (T8) Polyclonal Antibody.

Fig.1. Western Blot analysis of various cells using Phospho-Smad2/3 (T8) Polyclonal Antibody.

Fig.2. Western Blot analysis of VEC cells using Phospho-Smad2/3 (T8) Polyclonal Antibody.

Fig.2. Western Blot analysis of VEC cells using Phospho-Smad2/3 (T8) Polyclonal Antibody.

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