| Product name | Ribosomal Protein L7 Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the C-terminal region of human Ribosomal Protein L7 at AA range: 180-260 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat,Cat |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:10000;IF 1:50-200 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | RPL7; 60S ribosomal protein L7 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 32kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL7 encodes a ribosomal protein that is a component of the 60S subunit. 60S ribosomal protein L7 belongs to the L30P family of ribosomal proteins. It contains an N-terminal basic region-leucine zipper (BZIP)-like domain and the RNP consensus submotif RNP2. In vitro the BZIP-like domain mediates homodimerization and stable binding to DNA and RNA, with a preference for 28S rRNA and mRNA. The protein can inhibit cell-free translation of mRNAs, suggesting that it plays a regulatory role in the translation apparatus. It is located in the cytoplasm. The protein has been shown to be an autoantigen in patients with systemic autoimmune diseases, such as systemic lupus erythematosus. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. |
| Gene ID | 6129 |
| Alternative | RPL7; 60S ribosomal protein L7 |
| Others | Ribosomal Protein L7 Polyclonal Antibody detects endogenous levels of Ribosomal Protein L7 protein. |
| Accession | P18124 |
| Observed Band(KD) | 32 |
Fig.1. Western Blot analysis of various cells using Ribosomal Protein L7 Polyclonal Antibody diluted at 1:2000.
Fig.2. Western Blot analysis of customer's (cat sample) using Ribosomal Protein L7 Polyclonal Antibody. Antibody was diluted at 1:2000.
Fig.3. Western blot analysis of Mouse-kidney, Mouse-brain, hela, KB, SH-SY5Y, 293T, 3T3 lysates using Ribosomal Protein L7 antibody. Antibody was diluted at 1:2000.
You must be logged in to post a review.
Reviews
There are no reviews yet.