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RGS14 Polyclonal Antibody

RGS14 Polyclonal Antibody

Views(4) Publications(0) Catalog no(ABP53529)
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Specification

Product name RGS14 Polyclonal Antibody
Immunogen Synthesized peptide derived from the Internal region of human RGS14 at AA rangle: 100-180
Host Rabbit
Reactivity Human,Mouse,Rat
Applications WB,ELISA,IHC
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000;IHC 1:50-300;ELISA 1:2000-20000
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative RGS14; Regulator of G-protein signaling 14; RGS14

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 65kD
Storage buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background RGS14 encodes a member of the regulator of G-protein signaling family. Regulator of G-protein signaling 14 contains one RGS domain, two Raf-like Ras-binding domains (RBDs), and one GoLoco domain. The protein attenuates the signaling activity of G-proteins by binding, through its GoLoco domain, to specific types of activated, GTP-bound G alpha subunits. Acting as a GTPase activating protein (GAP), the protein increases the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.
Gene ID 10636
Alternative RGS14; Regulator of G-protein signaling 14; RGS14
Others RGS14 Polyclonal Antibody detects endogenous levels of RGS14 protein.
Accession O43566
Observed Band(KD) 65

Image & description

Fig. Western Blot analysis of 3T3 cells using RGS14 Polyclonal Antibody.

Fig. Western Blot analysis of 3T3 cells using RGS14 Polyclonal Antibody.

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