| Product name | Renilla Luciferase Reporter Gene Assay Kit |
| SampleType | Cells, Protoplasts and Plant leaves |
| Alternative | Renilla Luciferase |
| Kit components | • Renilla Luciferase Lysis Buffer (5×) • Renilla Luciferase Assay Buffer • Renilla Luciferase Substrate (50×) |
| Features & Benefits | The verified samples are complete in type and easy to operate． |
| Usage notes | • If not assayed immediately, samples can be stored at -80°C for one month.• It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.• Fresh samples are necessary for good results. If don't perform the assay at the same time, it's better to complete the Sample Preparation step before storing the samples. |
| Storage instructions | Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | In the presence of oxygen, Renilla luciferase can catalyze the oxidation of coelenterazine to coelenteramide, and light signals are also generated during the oxidation of coelenterine. Through the Bioluminescence system of Luciferase and its substrate, gene expression can be detected very sensitively and efficiently. Usually, the Transcriptional regulation element or 5'-promoter region of the gene of interest is cloned upstream of Luciferase, or the 3'- UTR region is cloned downstream of Luciferase to construct a Reporter gene plasmid, and then transfect the cell, treat the cell with appropriate drugs, and then Lytic cells. The Transcriptional regulation effect of drug treatment on the target gene is judged by detecting the activity of Luciferase. This kit has the characteristics of rapid detection, high sensitivity, wide detection range and no interference from intracellular activity. |
| Alternative | Renilla Luciferase |
Fig.Renilla Luciferase Reporter Gene Assay Kit
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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