| Product name | Rb (Acetyl-K873/K874) Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from part region of human Rb (Acetyl-K873/K874) protein at amino acid sequence of K873/872 |
| Host | Rabbit |
| Reactivity | Human:K873+K874,Mouse:K866+K867,Rat:K865+K866 |
| Applications | WB,IHC |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000;IHC 1:50-300 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | The protein encoded by RB1 is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in RB1 are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. RB1 (RB Transcriptional Corepressor 1) is a Protein Coding gene. Diseases associated with RB1 include Retinoblastoma and Small Cell Cancer Of The Lung, Somatic. Among its related pathways are Arrhythmogenic right ventricular cardiomyopathy (ARVC) and DNA Damage. |
| Gene ID | 5925 |
| Alternative | Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110 |
| Others | The antibody detects endogenous levels of Rb when Acetyl occurs at Lys873/874(human), Lys866/867(mouse) or Lys865/866(rat), while it doesn't react with total protein. |
| Accession | P06400 |
Fig.1. Western Blot analysis of 1, Mouse-heart 3,Mouse-brain cells using primary antibody diluted at 1:1000 (4°C overnight). Goat Anti-rabbit IgG Dylight 800 (Cat #: A23920) secondary antibody was diluted at 1:5000 at 25°C for 1 hour.
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