|Product name||Rat Secretory immunoglobulin A (sIgA) ELISA Kit|
|Applications notes||This Rat Secretory immunoglobulin A (sIgA) ELISA Kit employs a two-site sandwich ELISA to quantitate sIgA in samples. An antibody specific for sIgA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anysIgA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for sIgA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of sIgA bound in the initial step. The color development is stopped and the intensity of the color is measured.|
|SampleType||Cell culture supernatants, Other biological fluids, Plasma, Serum|
|Assay type||Sandwich ELISA (quantitative)|
|Assay duration||Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.|
|Kit components||• Rat Secretory immunoglobulin A microplate
• Rat Secretory immunoglobulin A standard
• Rat Secretory immunoglobulin A detect antibody
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
|Features & Benefits||Rat Secretory immunoglobulin A (sIgA) ELISA Kit has high sensitivity and excellent specificity for detection of Rat sIgA. No significant cross-reactivity or interference between Rat sIgA and analogues was observed.|
|Calibration range||Please inquire|
|Limit of detection||Please inquire|
|Usage notes||• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
|Storage instructions||The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||Secretory IgA (IgA) consists of two IgA monomers joined by the J-chain and furthermore a secretory component. It is secreted in plasma cells based in the lamina propia of mucosal membranes. Synthesis of IgA is independent from the synthesis of serum IgA. This means lack of serum IgA does not necessarily mean a lack of IgA. IgA is found in secretions in a specific form called secretory IgA', Secretory IgA is the major immunoglobulin in saliva, tears, colostrum, nasal mucous, mother´s milk, tracheobronchial and gastrointestinal secretes. It plays a major role in preventing adherence of microorganisms to mucosal sites, in activation of the alternative complement pathway and in activating inflammatory reactions. Newborns are provided with IgA by mother´s milk and are passively immunized against gastrointestinal infections.|
Fig.1. Rat Secretory immunoglobulin A (sIgA) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.