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Rat Protein Wnt-5a (WNT5A) ELISA Kit

Rat Protein Wnt-5a (WNT5A) ELISA Kit

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Product name Rat Protein Wnt-5a (WNT5A) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Protein Wnt-5a (WNT5A) ELISA Kit employs a two-site sandwich ELISA to quantitate WNT5A in samples. An antibody specific for WNT5A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyWNT5A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WNT5A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WNT5A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative WNT5A; hWNT5A; WNT-5A protein

Product Properties

Kit components • Rat Protein Wnt-5a microplate
• Rat Protein Wnt-5a standard
• Rat Protein Wnt-5a detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Protein Wnt-5a (WNT5A) ELISA Kit has high sensitivity and excellent specificity for detection of Rat WNT5A. No significant cross-reactivity or interference between Rat WNT5A and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 94% and 80% amino acid identity to the mouse Wnt5b protein and the human WNT5A protein, respectively. Alternative splicing of this gene generates 2 transcript variants. WNT5B encodes a deduced 359-amino acid protein with an N-terminal signal peptide, 4 N-linked glycosylation sites, and conserved residues of the WNT family. The WNT5B protein shares 80% sequence identity with WNT5A.
Gene ID 100009381
Alternative WNT5A; hWNT5A; WNT-5A protein
Accession Q27Q52

Image & description

Fig.1. Rat Protein Wnt-5a (WNT5A) Standard Curve.

Fig.1. Rat Protein Wnt-5a (WNT5A) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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