|Product name||Rat N-terminal procollagen III propeptide (PIIINP) ELISA Kit|
|Applications notes||This Rat N-terminal procollagen III propeptide (PIIINP) ELISA Kit employs a two-site sandwich ELISA to quantitate PIIINP in samples. An antibody specific for PIIINP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPIIINP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PIIINP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PIIINP bound in the initial step. The color development is stopped and the intensity of the color is measured.|
|SampleType||Cell culture supernatants, Other biological fluids, Plasma, Serum|
|Assay type||Sandwich ELISA (quantitative)|
|Assay duration||Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.|
|Kit components||• Rat N-terminal procollagen III propeptide microplate
• Rat N-terminal procollagen III propeptide standard
• Rat N-terminal procollagen III propeptide detect antibody
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
|Features & Benefits||Rat N-terminal procollagen III propeptide (PIIINP) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PIIINP. No significant cross-reactivity or interference between Rat PIIINP and analogues was observed.|
|Calibration range||Please inquire|
|Limit of detection||Please inquire|
|Usage notes||• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
|Storage instructions||The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||Plasma levels of PIIINP a marker of extracellular matrix metabolism activity have been linked to abdominal aortic aneurysm growth rate, but its relationship with thoracic aortic aneurysm (TAA) growth has never previously been studied. The PIIINP molecule is an indicator of both the synthesis and the degradation of type III collagen. Occasionally, the removal of PIIINP from the newly-synthesized type III collagen is not complete and the propeptide may remain attached to some of the molecules, known as type IIIpN-collagen. PIIINP is a degradation product of type IIIpN collagen. PIIINP has a molecular weight of 42 000 and contains three distinct domains: a triple-helical domain (Col 3) in the middle of the molecule, the Col 1-domain at the aminoterminal and Col 2-domain at the carboxyterminal end of the propeptide. PIIINP is cleared from the circulation by scavenger receptors in liver endothelial cells.|
Fig.1. Rat N-terminal procollagen III propeptide (PIIINP) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.