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Rat Kallikrein-6 (KLK6) ELISA Kit

Rat Kallikrein-6 (KLK6) ELISA Kit

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Specification

Product name Rat Kallikrein-6 (KLK6) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Kallikrein-6 (KLK6) ELISA Kit employs a two-site sandwich ELISA to quantitate KLK6 in samples. An antibody specific for KLK6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKLK6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KLK6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KLK6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative KLK6; Bssp; Klk7; MGC9355; NEUROSIN; PRSS18; PRSS9; SP59; ZYME; hK6; kallikrein 6 (neurosin; zyme); protease M; protease; serine; 18; protease; serine; 9

Product Properties

Kit components • Rat Kallikrein-6 microplate
• Rat Kallikrein-6 standard
• Rat Kallikrein-6 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Kallikrein-6 (KLK6) ELISA Kit has high sensitivity and excellent specificity for detection of Rat KLK6. No significant cross-reactivity or interference between Rat KLK6 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Kallikrein 6, also known as Zyme, Neurosin, PRSS9, myelencephalon-specific protease (MSP) and protease M, is a trypsin-like serine proteinase. Kallikrein 6 was originally described from the brain as an enzyme involved in degradation of amyloid plaque protein (APP) and hK6 was thought to be a beta secretase. Kallikrein 6 was shown to be elevated in the sera patients with Alzheimer’s disease and Parkinson’s disease and in animal models of multiple sclerosis. The neuronal protein alpha-synuclein was shown to be cleaved by hK6, as has APP and hK6 has been implicated in vascular morphogenesis and angiogenesis. MSP has also been shown to rapidly degrade myelin-specific protein, laminin and fibronectin.
Alternative KLK6; Bssp; Klk7; MGC9355; NEUROSIN; PRSS18; PRSS9; SP59; ZYME; hK6; kallikrein 6 (neurosin; zyme); protease M; protease; serine; 18; protease; serine; 9
Accession P36374

Image & description

Fig.1. Rat Kallikrein-6 (KLK6) Standard Curve.

Fig.1. Rat Kallikrein-6 (KLK6) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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