| Product name | Rat CNTF Protein |
| Sequence | Amino acid sequence derived from the processed form of Rat-CNTF (P20294)(Met 1-Met 200) was expressed |
| Applications | No tag |
| Applications notes | Rat |
| Activity | Measured by its ability to induce proliferation in TF-1 cells. The ED50 for this effect is 0.02748 ng/mL. |
| Protein length | The recombinant Rat CNTF consisting of 200 amino acids and has a calculated molecular mass of 22.9 kDa.It migrates as an approximately 24 kDa band in SDS-PAGE under reducing conditions. |
| Preparation method | E. coli |
| Purity | > 97 % as determined by SDS-PAGE |
| Alternative | Ciliary neurotrophic factor;CNTF;H-CNTF |
| Formulation | Liquid in from sterile 20 mM Tris 50mM NaCl ,pH 8.0 |
| Features & Benefits | Endotoxin: <1 EU per μg of the protein as determined by the LAL method. |
| Molecular weight | 22.9 kDa |
| Usage notes | Always centrifuge tubes before opening. It is recommended to diluted the protein using the PBS buffer or Protein storage solution to working concentration |
| Storage instructions | The Rat-CNTF protein product should be stored desiccated below -20°C. The protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Ciliary neurotrophic factor (CNTF) is a member of the cytokine family. It is a polypeptide hormone that has the function of promoting the synthesis of neurotransmitters and the growth of neurites in a specific neuronal population. Its action seems to be limited to the nervous system. Ciliary neurotrophic factor (CNTF) exerts its biological effects by activating a multi-subunit receptor complex consisting of an extracellular CNTF-binding subunit (CNTFα) and two transmembrane signaling proteins (glycoprotein gp130 and LIF receptor). CNTF is thought to be a potent survival factor in neurons and oligodendrocytes and may be associated with reduced tissue destruction during inflammatory episodes. CNTF is also a survival factor for peripheral sensory sympathetic ganglions and ciliary ganglia neurons. CNTF has been reported to have therapeutic potential and to greatly differentiate. |
| Gene ID | 25707 |
| Alternative | Ciliary neurotrophic factor;CNTF;H-CNTF |
| Accession | P20294 |
Fig.SDS-PAGE analysis of Rat CNTF Protein
You must be logged in to post a review.
Reviews
There are no reviews yet.