| Product name | Rat bFGF protein |
| Sequence | Amino acid sequence derived from Rat FGF2 isoform (P13109) (Ala11-Ser154) was expressed. |
| Activity | Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.6 ng/mL. |
| Protein length | The recombinant Rat bFGF protein consists of 145 amino acids (N-Met) and has a predicted molecular mass of 16.2 KD. The apparent molecular mass of the Rat FGF2 is approximately 16.2 KD in SDS-PAGE under reducing conditions due to glycosylation. |
| Preparation method | E. coli |
| Purity | >95% as determined by SDS-PAGE. |
| Alternative | Fibroblast Growth Factor 2; FGF-2; Basic Fibroblast Growth Factor; bFGF; Heparin-Binding Growth Factor 2; HBGF-2; FGF2; FGFB |
| Formulation | Lyophilized from sterile 20 mM Tris , 150 mM NaCl, pH8.0. |
| Features & Benefits | Endotoxin: < 1.0 EU per µg of the protein as determined by the LAL method. |
| Molecular weight | 16.2 KD |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Rat bFGF protein using the buffer we provided not less than 100 µg/mL, which can then be further diluted to other aqueous solutions. |
| Storage instructions | Lyophilized Rat bFGF proteinshould be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.100% HSA or BSA). Please prevent freeze-thaw cycles. |
| Shipping | The product is shipped at ambient temperature. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Basic fibroblast growth factor (bFGF), also known as FGF2, is a member of the fibroblast growth factor (FGF) family. FGF basic modulates such normal processes as angiogenesis, wound healing and tissue repair, embryonic development and differentiation, neuronal function and neural degeneration. Transgenic over-expression of FGF basic results in excessive proliferation and angiogenesis reminiscent of a variety of pathological conditions. |
| Gene ID | 54250 |
| Alternative | Fibroblast Growth Factor 2; FGF-2; Basic Fibroblast Growth Factor; bFGF; Heparin-Binding Growth Factor 2; HBGF-2; FGF2; FGFB |
| Accession | P13109 |
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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