Login Register
English
0

Cart

$ 0

Rat Activated CDC42 kinase 1 (TNK2) ELISA Kit

Rat Activated CDC42 kinase 1 (TNK2) ELISA Kit

Views(236) Publications(0) Catalog no(KTE100064)
Datasheet Print Share Email Share
  • Specification
  • FAQ
  • Publications(0)
  • Comments(0)

Specification

Product name Rat Activated CDC42 kinase 1 (TNK2) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Activated CDC42 kinase 1 (TNK2) ELISA Kit employs a two-site sandwich ELISA to quantitate TNK2 in samples. An antibody specific for TNK2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNK2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNK2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNK2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TNK2; ACK; ACK1; FLJ44758; FLJ45547; p21cdc42Hs; activated Cdc42-associated kinase 1; activated p21cdc42Hs kinase

Product Properties

Kit components • Rat Activated CDC42 kinase 1 microplate
• Rat Activated CDC42 kinase 1 standard
• Rat Activated CDC42 kinase 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Activated CDC42 kinase 1 (TNK2) ELISA Kit has high sensitivity and excellent specificity for detection of Rat TNK2. No significant cross-reactivity or interference between Rat TNK2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Activated CDC42 kinase 1 is a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined.
Gene ID 303882
Alternative TNK2; ACK; ACK1; FLJ44758; FLJ45547; p21cdc42Hs; activated Cdc42-associated kinase 1; activated p21cdc42Hs kinase
Accession Q5U2X5

Image & description

Fig.1. Rat Activated CDC42 kinase 1 (TNK2) Standard Curve.

Fig.1. Rat Activated CDC42 kinase 1 (TNK2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

This product has been cited in0publications

Reviews

There are no reviews yet.

Be the first to review “Rat Activated CDC42 kinase 1 (TNK2) ELISA Kit”