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Rat 14-3-3 protein theta (YWHAQ) ELISA Kit

Rat 14-3-3 protein theta (YWHAQ) ELISA Kit

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Specification

Product name Rat 14-3-3 protein theta (YWHAQ) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat 14-3-3 protein theta (YWHAQ) ELISA Kit employs a two-site sandwich ELISA to quantitate YWHAQ in samples. An antibody specific for YWHAQ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyYWHAQ present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for YWHAQ is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of YWHAQ bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative YWHAQ; 14-3-3; 1C5; HS1; 14-3-3 protein T-cell; 14-3-3 protein tau; 14-3-3 protein theta; OTTHUMP00000165887; protein tau; tyrosine 3/tryptophan 5 -monooxygenase activation protein; theta polypeptide

Product Properties

Kit components • Rat 14-3-3 protein theta microplate
• Rat 14-3-3 protein theta standard
• Rat 14-3-3 protein theta detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat 14-3-3 protein theta (YWHAQ) ELISA Kit has high sensitivity and excellent specificity for detection of Rat YWHAQ. No significant cross-reactivity or interference between Rat YWHAQ and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background YWHAq product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease.The deduced 245-amino acid protein has a calculated molecular mass of 28 kD and shares 72% amino acid identity with bovine brain 14-3-3-eta. Northern blot analysis of Jurkat and HeLa cells showed strong bands at 1.9 and 2.1 kb, and a weaker band at 1.7 kb.
Gene ID 100008930
Alternative YWHAQ; 14-3-3; 1C5; HS1; 14-3-3 protein T-cell; 14-3-3 protein tau; 14-3-3 protein theta; OTTHUMP00000165887; protein tau; tyrosine 3/tryptophan 5 -monooxygenase activation protein; theta polypeptide
Accession Q6Q6X0

Image & description

Fig.1. Rat 14-3-3 protein theta (YWHAQ) Standard Curve.

Fig.1. Rat 14-3-3 protein theta (YWHAQ) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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