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Raf-B (phospho Ser446) Polyclonal Antibody

Raf-B (phospho Ser446) Polyclonal Antibody

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Specification

Product name Raf-B (phospho Ser446) Polyclonal Antibody
Immunogen Synthesized peptide derived from human Raf-B around the phosphorylation site of S446
Host Rabbit
Reactivity Human, Mouse
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative BRAF; BRAF1; RAFB1; Serine/threonine-protein kinase B-raf; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background BRAF (B-Raf proto-oncogene, serine/threonine kinase) encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in BRAF are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in BRAF have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for BRAF.
Gene ID 673
Alternative BRAF; BRAF1; RAFB1; Serine/threonine-protein kinase B-raf; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1
Others Phospho-Raf-B (S446) Polyclonal Antibody detects endogenous levels of Raf-B protein only when phosphorylated at S446.
Accession P15056
Observed Band(KD) 110

Image & description

Fig.1. Western Blot analysis of 293 cells using Phospho-Raf-B (S446) Polyclonal Antibody diluted at 1:1000.

Fig.1. Western Blot analysis of 293 cells using Phospho-Raf-B (S446) Polyclonal Antibody diluted at 1:1000.

Fig.2. Western blot analysis of K562 293T lysis using Phospho-Raf-B (S446) antibody. Antibody was diluted at 1:1000.

Fig.2. Western blot analysis of K562 293T lysis using Phospho-Raf-B (S446) antibody. Antibody was diluted at 1:1000.

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