Product Information

• Product name

  Raf-B (phospho Ser446) Polyclonal Antibody

• Immunogen

  Synthesized peptide derived from human Raf-B around the phosphorylation site of S446

• Host

  Rabbit

• Reactivity

  Human, Mouse

• Applications

  ELISA, IHC-P, WB

• Application notes

  Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.

• Clonality

  Polyclonal

• Isotype

  Rabbit IgG

• Purification

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Fig.1. Western Blot analysis of 293 cells using Phospho-Raf-B (S446) Polyclonal Antibody diluted at 1:1000.

Fig.2. Western blot analysis of K562 293T lysis using Phospho-Raf-B (S446) antibody. Antibody was diluted at 1:1000.

Product Properties

• Formulation

  Liquid solution

• Concentration

  1 mg/ml

• Storage buffer

  PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.

• Storage instructions

  Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

• Shipping

  Gel pack with blue ice.

• Precautions

  The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

• Background

  BRAF (B-Raf proto-oncogene, serine/threonine kinase) encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in BRAF are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in BRAF have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for BRAF.

• Gene ID

  673

• Alternative names

  BRAF; BRAF1; RAFB1; Serine/threonine-protein kinase B-raf; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1

• Others

  Phospho-Raf-B (S446) Polyclonal Antibody detects endogenous levels of Raf-B protein only when phosphorylated at S446.

• Accession

  P15056