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Raf-1 (phospho Ser301) Polyclonal Antibody

Raf-1 (phospho Ser301) Polyclonal Antibody

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Specification

Product name Raf-1 (phospho Ser301) Polyclonal Antibody
Immunogen Synthesized peptide derived from human Raf-1 around the phosphorylation site of S301
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:10000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative RAF1; RAF; RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background RAF1 (Raf-1 proto-oncogene, serine/threonine kinase) is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in RAF1 are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
Gene ID 5894
Alternative RAF1; RAF; RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1
Others Phospho-Raf-1 (S301) Polyclonal Antibody detects endogenous levels of Raf-1 protein only when phosphorylated at S301.
Accession P04049
Observed Band(KD) 75

Image & description

Fig.1. Western Blot analysis of A549 cells using Phospho-Raf-1(S301) Polyclonal Antibody diluted at 1:1000.

Fig.1. Western Blot analysis of A549 cells using Phospho-Raf-1(S301) Polyclonal Antibody diluted at 1:1000.

Fig.2. The picture was kindly provided by our customer,antibody was diluted at 1:500.

Fig.2. The picture was kindly provided by our customer,antibody was diluted at 1:500.

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