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Rad52 Polyclonal Antibody

Rad52 Polyclonal Antibody

Views(293) Publications(0) Catalog no(ABP52310)
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Specification

Product name Rad52 Polyclonal Antibody
Immunogen Synthesized peptide derived from human Rad52 around the non-phosphorylation site of Y104
Host Rabbit
Reactivity Human, Mouse
Applications ELISA, IF, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:10000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative RAD52; DNA repair protein RAD52 homolog

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background RAD52 homolog, DNA repair protein encoded by RAD52 shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. RAD52 product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. A pseudogene of RAD52 is present on chromosome 2. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of RAD52 have been described, but their full-length nature is not known.
Gene ID 5893
Alternative RAD52; DNA repair protein RAD52 homolog
Others Rad52 Polyclonal Antibody detects endogenous levels of Rad52 protein.
Accession P43351
Observed Band(KD) 46

Image & description

Fig.1. Western Blot analysis of various cells using Rad52 Polyclonal Antibody.

Fig.1. Western Blot analysis of various cells using Rad52 Polyclonal Antibody.

Fig.2. Western Blot analysis of 293 cells using Rad52 Polyclonal Antibody.

Fig.2. Western Blot analysis of 293 cells using Rad52 Polyclonal Antibody.

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