Product name | PurKine™ Strep II-Tag Strep-Tactin Resin 4FF |
Applications notes | PurKine™ Strep II-Tag Strep-Tactin Resin 4FF effectively purifies high levels of overexpressed Strep II-tagged fusion proteins in gravity column procedures at a variety of scales. The Resin consists of 90μm beads of cross-linked 4% agarose, to which Strep-Tactin has been coupled. Tests confirm that performance equals or exceeds popular Strep II-Tag Purification Resins from other suppliers, and no decrease in performance occurs after at least five repeated uses. |
Alternative | Strep-Tactin ; Strep II-Tag resin; Strep-Tactin resin; Strep II resin |
Formulation | Liquid solution with 50% slurry in 20% ethanol. |
Features & Benefits | • High capacity—6 mg of Strep II tag fusion protein per mL of resin • High performance—Strep-Tactin is one of the most stable proteins . • Cost-effective—No performance decrease after at least five repeated uses of the same batch of resin. • Flexible—available in multiple formats including bulk resin, spin columns and complete kits. • Easy to use— Pre-formulated buffers available for kit formats. • Robust—highly crosslinked beads tolerate linear flow rates up to 300 cm/hour. |
Storage buffer | Liquid in PBS buffer containing 20% ethanol. |
Storage instructions | Stable for one year at 2-8°C from date of shipment. Do not freeze. |
Shipping | Blue ice |
Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
Background | PurKine ™ Strep II Purification system is based on innovative high-capacity matrix for convenient single-step purification of biotin and biotinylated substances. It’s based on innovative high loading matrix, which can easily purify strep II labeled protein in one step. Strep II is a short peptide (8 amino acids). The binding affinity of strep tag II to strep tactin is 100 times higher than the streptavidin. This product mix optimizes the process to achieve optimal protein yield, stability and solubility. It,s also confirm that no decrease in performance occurs after at least five repeated uses. The PurKine™ Strep II Purification resin is also available as prepacked spin column and kit formats. |
Alternative | Strep-Tactin ; Strep II-Tag resin; Strep-Tactin resin; Strep II resin |
Fig.1. Abbkine PurKine™ is series of unique purification portfolio from settled resin, prepacked spin columns and full kits for your most types of sample purification and preparation.
Fig.2. Rather than traditional method of resin production, Abbkine is taking advantage of the latest synthetics process with cutting-edge techniques for the resin development and accessories optimization. PurKine™ tools are available for your samples from fusion proteins, nucleic acids, antibodies and biomolecule markers with improved productivity, simplified workflow and cost saving.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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