| Product name | PurKine™ Biotin-Tag Streptavidin Resin 6FF |
| Applications notes | PurKine™ Biotin-Tag Streptavidin Packed Column effectively purifies high levels of biotin and biotinylated substances in gravity column procedures at a variety of scales. The Resin consists of 90μm beads of cross-linked 6% agarose, to which Streptavidin has been coupled. Tests confirm that performance equals or exceeds popular Streptavidin resins from other suppliers, and no decrease in performance occurs after at least five repeated uses. In addition, its high flow properties make it excellent for scaling-up. |
| Alternative | Streptavidin ; Biotin resin; Streptavidin resin; Biotin tag resin |
| Formulation | Liquid solution with 50% slurry in 20% ethanol. |
| Features & Benefits | • High capacity—1 to 3 mg of biotinylated BSA per mL of resin.More than 120 nmol Biotin per mL of resin • High performance—Streptvidin has a low isoelectric point (pI=5) and no carbohydrate groups, resulting in low nonspecific binding. • Cost-effective—No performance decrease after at least five repeated uses of the same batch of resin. • Flexible—available in multiple formats including bulk resin, spin columns and complete kits. • Robust—highly crosslinked beads tolerate linear flow rates up to 500 cm/hour. |
| Storage buffer | Liquid in PBS buffer containing 20% ethanol. |
| Storage instructions | Stable for one year at 2-8°C from date of shipment. Do not freeze. |
| Shipping | Blue ice |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | PurKine™ Biotin-Tag Purification system is based on innovative high-capacity matrix for convenient single-step purification of biotin and biotinylated substances. Biotinylated antibodies, proteins, peptides, nucleic acids and other molecules or interaction complexes can be captured, immunoprecipitated, removed or purified from samples using this streptavidin resin. The portfolio allow optimization of process for maximum protein yield, stability and solubility. Tests also confirm that no decrease in performance occurs after at least five repeated uses. The PurKine™ Biotin-Tag Purification resin is also available as prepacked spin column and kit formats. |
| Alternative | Streptavidin ; Biotin resin; Streptavidin resin; Biotin tag resin |
Fig.1. Abbkine PurKine™ is series of unique purification portfolio from settled resin, prepacked spin columns and full kits for your most types of sample purification and preparation.
Fig.2. Rather than traditional method of resin production, Abbkine is taking advantage of the latest synthetics process with cutting-edge techniques for the resin development and accessories optimization. PurKine™ tools are available for your samples from fusion proteins, nucleic acids, antibodies and biomolecule markers with improved productivity, simplified workflow and cost saving.
Author：Han, Hongjia, et al. Publication name：Molecular Plant Pathology IF：4.9
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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