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Protein Quantification Kit (Bradford Assay)

Protein Quantification Kit (Bradford Assay)

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Product name Protein Quantification Kit (Bradford Assay)
Applications notes Abbkine Protein Quantification Kit (Bradford Assay) provides a simple and rapid procedure for determining the concentration of protein in samples. The method utilizes an improved Coomassie blue G reagent which forms a blue complex in the presence of protein. When coomassie dye binds protein in an acidic medium, an immediate shift in absorption maximum occurs from 465nm to 595nm with a concomitant color change from brown to blue. The intensity of the blue complex is proportional to the amount of protein in the sample.
Detection method Colorimetric
Alternative Coomassie

Product Properties

Kit components • Coomassie Assay Reagent
• Albumin Standard (BSA)
Features & Benefits • Time saving—almost immediate color development.
• Compatible—unaffected by most chemical substances. The concentration of mercaptoethanol and dithiothreitol in the samples can reach 1mM and 5 mM respectively.
• Broad range—working range from 50 to 1000 µg/ml.
Calibration range 50-1000 µg/ml
Limit of detection 25 µg/ml
Usage notes Due to the influence of high concentration detergent, it is necessary to ensure that the concentration of SDS is lower than 0.1 %, Triton X-100 is lower than 0.1 % and Tween 20, 60, 80 is lower than 0.06 % in the sample.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The Bradford assay, a colorimetric protein assay, is based on an absorbance shift of the dye Coomassie Brilliant Blue G-250. The Coomassie Brilliant Blue G-250 dye exists in three forms: anionic (blue), neutral (green), and cationic (red). Under acidic conditions, the red form of the dye is converted into its blue form, binding to the protein being assayed. If there's no protein to bind, then the solution will remain brown. The cationic (unbound) form is green / red and has an absorption spectrum maximum historically held to be at 465 nm. The anionic bound form of the dye which is held together by hydrophobic and ionic interactions, has an absorption spectrum maximum historically held to be at 595 nm. The increase of absorbance at 595 nm is proportional to the amount of bound dye, and thus to the amount (concentration) of protein present in the sample.
Alternative Coomassie

Image & description

Fig. Protein Quantification Kit (Bradford Assay) standard curve

Fig. Protein Quantification Kit (Bradford Assay) standard curve

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