- Product name
Protein Quantification Kit (BCA Assay)
- Application notes
Abbkine Protein Quantification Kit (BCA Assay) provides a simple, rapid, detergent-compatible procedure for the colorimetric detection and quantitation of total protein. The method utilizes the reduction of the copper cation (Cu2+) to the cuprous cation (Cu+) by protein in an alkaline medium. The generated Cu+ forms an intensely purple-colored complex with the bicinchoninic acid (BSA) with a very strong absorbance at 562nm. The intensity of the water-soluble complex is proportional to the amount of protein in the sample. The BCA Protein Assay is suitable for measuring protein concentration in the range of 50-1000µg/ml.
- Detection method
- Kit components
• BCA Reagent A (2x) • BCA Reagent B • BSA Standard
- Features & Benefits
• Time saving—much easier and four times faster than the classical Lowry method. • Sensitive—detect down to 25 µg/ml and the minimum amount of detection protein reaches 0.5μg. • Compatible—unaffected by most ionic and nonionic detergents. Can be compatible with up to 5 % SDS, 5 % Triton X-100, 5 % Tween-20, 60, 80 in the sample. • Good linearity—linear working range from 50 to 1000 µg/ml. • Excellent uniformity— exhibits less protein-to-protein variation than dye-binding methods.
- Calibration range
- Limit of detection
- Usage notes
• The color of the reaction is not only related to the protein concentration of the sample, but also to the temperature of the reaction. If the concentration of protein in the sample is high (＞50μg/ml), the reaction temperature is usually 37℃. If the concentration of protein in the sample is low (< 50μg/ml), the reaction temperature is 60℃. At this temperature, tryptophan, tyrosine and peptide bonds are fully oxidized, greatly improving the detection sensitivity.
• Commonly used concentrations of detergent SDS, Triton X-100, Tween do not affect the detection results, but results are affected by chelating agents (EDTA, EGTA), reducing agents (DTT, mercaptoethanol) and lipids. If such substance exists in the sample, try the Bradford method.
- Storage instructions
The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Gel pack with blue ice.
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Bicinchoninic Acid (BCA) is capable of forming an intense purple complex with cuprous ion, Cu+, in an alkaline environment. Cu+ is produced from the reaction of protein with alkaline Cu2+. The resulting reaction and color produced is the basis for a common protein quantification method capable of measuring protein concentration over a wide range. Increasing protein concentrations produce proportionally deeper colors. The BCA protein assay demonstrates higher tolerances towards common interfering substances, such as nonionic detergents and buffer salts than the Lowry technique.
- Alternative names
Bicinchoninic acid protein
Most popular with customers
Protein Quantification Kit (BCA Assay)
Click to download booklet for more details
Notice:Protocol may vary from different lot which is subject to the manual along with the shipment. Please contact email@example.com for your enquiry.
Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.
Preparation methods for Biochemical
Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.
Antibody application protocols
Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.
- ♦ Antibody Western Blotting (WB) protocol
- ♦ Antibody Immunohistochemistry (IHC) protocol
- ♦ Antibody Immunofluorescence (IF) protocol
- ♦ Antibody Immunoprecipitation (IP) protocol
- ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol
Protein&peptide usage suggestions
Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.
- ♦ Handling and storage suggestion for peptides and protein
- ♦ Cytokines and growth factors for cell culture application
Commonly used assay kits guidelines
Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.
We hope this will be helpful for your research work. Please let us know through firstname.lastname@example.org if you need more information or support.