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PP2A-Cα Polyclonal Antibody

PP2A-Cα Polyclonal Antibody

Views(616) Publications(0) Catalog no(ABP52249)
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Specification

Product name PP2A-Cα Polyclonal Antibody
Immunogen Synthesized peptide derived from human PP2A-Cα around the non-phosphorylation site of Y307
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IF, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:5000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative PPP2CA; Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform; PP2A-alpha; Replication protein C; RP-C

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background PPP2CA encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. PPP2CA encodes an alpha isoform of the catalytic subunit.
Gene ID 5515
Alternative PPP2CA; Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform; PP2A-alpha; Replication protein C; RP-C
Others PP2A-Cα Polyclonal Antibody detects endogenous levels of PP2A-Cα protein.
Accession P67775
Observed Band(KD) 35

Image & description

Fig.1. Western Blot analysis of various cells using PP2A-Cα Polyclonal Antibody diluted at 1:2000.

Fig.1. Western Blot analysis of various cells using PP2A-Cα Polyclonal Antibody diluted at 1:2000.

Fig.2. Western Blot analysis of A549 cells using PP2A-Cα Polyclonal Antibody diluted at 1:2000.

Fig.2. Western Blot analysis of A549 cells using PP2A-Cα Polyclonal Antibody diluted at 1:2000.

Fig.3. Western Blot analysis of various cells using Antibody diluted at 1:1000. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.3. Western Blot analysis of various cells using Antibody diluted at 1:1000. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

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