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Plant-actin Polyclonal Antibody

Plant-actin Polyclonal Antibody

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Specification

Product name Plant-actin Polyclonal Antibody
Immunogen Recombinant Protein
Host Rabbit
Reactivity Arabidopsis Nicotiana tabacum Oryza sativa Hordeum vulgare
Applications WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:3000).
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen
Alternative ACTB; Actin; cytoplasmic 1; Beta-actin

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Arabidopsis has a complex actin gene family that encodes six protein subclasses. One of the classes is represented by the proteins ACT2 and ACT8, which are similar proteins, but have different flanking sequences, introns and silent nucleotide positions in codons. ACT8 is expressed in root, stems, leaves, flowers, pollen and siliques.
Alternative ACTB; Actin; cytoplasmic 1; Beta-actin
Others The antibody detects endogenous plant-actin protein.
Observed Band(KD) 43

Image & description

Fig. Western blot analysis of Nicotiana tabacum leaf (1), Nicotiana tabacum root (2), Nicotiana seed (3), Oryza sativa flower (4), Oryza sativa seed (5), Oryza sativa leaf (6), Hordeum vulgare seed (7), Hordeum vulgare leaves (8), diluted at 1:3000. Secondary antibody was diluted at 1:20000.

Fig. Western blot analysis of Nicotiana tabacum leaf (1), Nicotiana tabacum root (2), Nicotiana seed (3), Oryza sativa flower (4), Oryza sativa seed (5), Oryza sativa leaf (6), Hordeum vulgare seed (7), Hordeum vulgare leaves (8), diluted at 1:3000. Secondary antibody was diluted at 1:20000.

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