| Product name | Plant-actin Polyclonal Antibody |
| Immunogen | Recombinant Protein |
| Host | Rabbit |
| Reactivity | Arabidopsis Nicotiana tabacum Oryza sativa Hordeum vulgare |
| Applications | WB |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:3000). |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen |
| Alternative | ACTB; Actin; cytoplasmic 1; Beta-actin |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Storage buffer | PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Arabidopsis has a complex actin gene family that encodes six protein subclasses. One of the classes is represented by the proteins ACT2 and ACT8, which are similar proteins, but have different flanking sequences, introns and silent nucleotide positions in codons. ACT8 is expressed in root, stems, leaves, flowers, pollen and siliques. |
| Alternative | ACTB; Actin; cytoplasmic 1; Beta-actin |
| Others | The antibody detects endogenous plant-actin protein. |
| Observed Band(KD) | 43 |
Fig. Western blot analysis of Nicotiana tabacum leaf (1), Nicotiana tabacum root (2), Nicotiana seed (3), Oryza sativa flower (4), Oryza sativa seed (5), Oryza sativa leaf (6), Hordeum vulgare seed (7), Hordeum vulgare leaves (8), diluted at 1:3000. Secondary antibody was diluted at 1:20000.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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