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Phosphotyrosine Monoclonal Antibody

Phosphotyrosine Monoclonal Antibody

Views(103) Publications(0) Catalog no(ABM40204)
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Specification

Product name Phosphotyrosine Monoclonal Antibody
Immunogen Purified Protein
Host Mouse
Reactivity All
Applications IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:1000-1:3000).
Clonality Monoclonal
Preparation method The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation. Its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.
Others The antibody detects Phosphotyrosine.
Accession N/A

Image & description

Fig.1. Western blot analysis of 1) Jurkat, 2) 293T, 3) Rat Brain Tissue, 4) Mouse Brain Tissue with Phosphotyrosine Mouse mAb diluted at 1:2000.

Fig.1. Western blot analysis of 1) Jurkat, 2) 293T, 3) Rat Brain Tissue, 4) Mouse Brain Tissue with Phosphotyrosine Mouse mAb diluted at 1:2000.

Fig.2. Immunohistochemical analysis of paraffin-embedded Human Breast Caricnoma using PhosphotyrosineMouse mAb diluted at 1:200.

Fig.2. Immunohistochemical analysis of paraffin-embedded Human Breast Caricnoma using PhosphotyrosineMouse mAb diluted at 1:200.

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