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PDE4B/C/D Polyclonal Antibody

PDE4B/C/D Polyclonal Antibody

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Specification

Product name PDE4B/C/D Polyclonal Antibody
Immunogen Synthesized peptide derived from human PDE4B/C/D around the non-phosphorylation site of S133/119/190
Host Rabbit
Reactivity Human,Mouse,Rat
Applications WB,IHC,IF,ELISA
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:10000;IF 1:50-200
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative PDE4B; DPDE4; cAMP-specific 3'; 5'-cyclic phosphodiesterase 4B; DPDE4; PDE32; PDE4C; DPDE1; cAMP-specific 3',5'-cyclic phosphodiesterase 4C; DPDE1; PDE21; PDE4D; DPDE3; cAMP-specific 3',5'-cyclic phosphodiesterase 4D; DPDE3; PDE43

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 76kD
Storage buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background PDE4D encodes one of four mammalian counterparts to the fruit fly 'dunce' gene. The encoded protein has 3',5'-cyclic-AMP phosphodiesterase activity and degrades cAMP, which acts as a signal transduction molecule in multiple cell types. PDE4D uses different promoters to generate multiple alternatively spliced transcript variants that encode functiona.
Gene ID 5144/5142/5143
Alternative PDE4B; DPDE4; cAMP-specific 3'; 5'-cyclic phosphodiesterase 4B; DPDE4; PDE32; PDE4C; DPDE1; cAMP-specific 3',5'-cyclic phosphodiesterase 4C; DPDE1; PDE21; PDE4D; DPDE3; cAMP-specific 3',5'-cyclic phosphodiesterase 4D; DPDE3; PDE43
Others PDE4B/C/D Polyclonal Antibody detects endogenous levels of PDE4B/C/D protein.
Accession Q07343/Q08493/Q08499
Observed Band(KD) 76

Image & description

Fig.1. Western Blot analysis of various cells using PDE4B/C/D Polyclonal Antibody.

Fig.1. Western Blot analysis of various cells using PDE4B/C/D Polyclonal Antibody.

Fig.2. Western Blot analysis of K562 cells using PDE4B/C/D Polyclonal Antibody.

Fig.2. Western Blot analysis of K562 cells using PDE4B/C/D Polyclonal Antibody.

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