Login Register
English
0

Cart

$ 0

PCNA Polyclonal Antibody

PCNA Polyclonal Antibody

Views(49) Publications(0) Catalog no(ABP0112)
Datasheet Print Share Email Share
  • Specification
  • FAQ
  • Publications(0)
  • Comments(0)

Specification

Product name PCNA Polyclonal Antibody
Immunogen Synthesized peptide derived from the Internal region of human PCNA at AA range: 30-110
Host Rabbit
Reactivity Human, Monkey, Mouse, Rat
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative PCNA; Proliferating cell nuclear antigen; PCNA; Cyclin

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 36 KD
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Proliferating cell nuclear antigen encoded by PCNA is found in the nucleus and is a cofactor of DNA polymerase delta. Proliferating cell nuclear antigen acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, proliferating cell nuclear antigen is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of PCNA have been described on chromosome 4 and on the X chromosome.
Gene ID 5111
Alternative PCNA; Proliferating cell nuclear antigen; PCNA; Cyclin
Others PCNA Polyclonal Antibody detects endogenous levels of PCNA protein.
Accession P12004

Image & description

Fig.1. Western Blot analysis of various cells using PCNA Polyclonal Antibody diluted at 1:2000.

Fig.1. Western Blot analysis of various cells using PCNA Polyclonal Antibody diluted at 1:2000.

Fig.2. Western Blot analysis of COS7 cells using PCNA Polyclonal Antibody diluted at 1:2000.

Fig.2. Western Blot analysis of COS7 cells using PCNA Polyclonal Antibody diluted at 1:2000.

This product has been cited in0publications

Reviews

There are no reviews yet.

Be the first to review “PCNA Polyclonal Antibody”