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PCBP1 Polyclonal Antibody

PCBP1 Polyclonal Antibody

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Specification

Product name PCBP1 Polyclonal Antibody
Immunogen Synthesized peptide derived from part region of human PCBP1 protein at amino acid sequence of 30-110
Host Rabbit
Reactivity Human,Mouse
Applications WB,ELISA
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000;ELISA 1:5000-20000
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative Poly(rC)-binding protein 1; Alpha-CP1; Heterogeneous nuclear ribonucleoprotein E1; Nucleic acid-binding protein SUB2.3; hnRNP E1

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 39kD
Storage buffer PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background This intronless gene is thought to have been generated by retrotransposition of a fully processed PCBP-2 mRNA. PCBP1 and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability.
Gene ID 5093
Alternative Poly(rC)-binding protein 1; Alpha-CP1; Heterogeneous nuclear ribonucleoprotein E1; Nucleic acid-binding protein SUB2.3; hnRNP E1
Others The antibody detects endogenous levels of PCBP1 protein
Accession Q15365
Observed Band(KD) 39

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