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PBK (phospho Thr9) Polyclonal Antibody

PBK (phospho Thr9) Polyclonal Antibody

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Specification

Product name PBK (phospho Thr9) Polyclonal Antibody
Immunogen Synthesized peptide derived from human PBK around the phosphorylation site of T9
Host Rabbit
Reactivity Human
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:40000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative PBK; TOPK; Lymphokine-activated killer T-cell-originated protein kinase; Cancer/testis antigen 84; CT84; MAPKK-like protein kinase; Nori-3; PDZ-binding kinase; Spermatogenesis-related protein kinase; SPK; T-LAK cell-originated protein kinas

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background PBK (PDZ binding kinase) encodes a serine/threonine protein kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. The encoded protein may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. Overexpression of PBK has been implicated in tumorigenesis. Alternative splicing results in multiple transcript variants.
Gene ID 55872
Alternative PBK; TOPK; Lymphokine-activated killer T-cell-originated protein kinase; Cancer/testis antigen 84; CT84; MAPKK-like protein kinase; Nori-3; PDZ-binding kinase; Spermatogenesis-related protein kinase; SPK; T-LAK cell-originated protein kinas
Others Phospho-PBK (T9) Polyclonal Antibody detects endogenous levels of PBK protein only when phosphorylated at T9.
Accession Q96KB5
Observed Band(KD) 36

Image & description

Fig. Western Blot analysis of 293T cells using Phospho-PBK (T9) Polyclonal Antibody diluted at 1:500.

Fig. Western Blot analysis of 293T cells using Phospho-PBK (T9) Polyclonal Antibody diluted at 1:500.

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