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PARP Monoclonal Antibody

PARP Monoclonal Antibody

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Specification

Product name PARP Monoclonal Antibody
Immunogen Synthetic Peptide
Host Mouse
Reactivity Chicken, Human
Applications WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:1000-1:3000).
Clonality Monoclonal
Preparation method The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 116 KD
Storage buffer PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background PARP1 encodes a chromatin-associated enzyme, poly (ADP-ribosyl)transferase, which modifies various nuclear proteins by poly (ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, poly (ADP-ribose) polymerase 1 may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.
Gene ID 142
Others The antibody detects endogenous PARP protein.
Accession P09874

Image & description

Fig. Western blot analysis of 1) Hela, 2) 293T, 3) Jurkat, diluted at 1:2000.

Fig. Western blot analysis of 1) Hela, 2) 293T, 3) Jurkat, diluted at 1:2000.

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