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Pan Methylated Lysine Monoclonal Antibody (Mix)

Pan Methylated Lysine Monoclonal Antibody (Mix)

Views(666) Publications(1) Catalog no(ABM0060)
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Specification

Product name Pan Methylated Lysine Monoclonal Antibody (Mix)
Immunogen Conjugated Protein
Host Mouse
Reactivity All
Applications IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:1000-1:2000), IHC-P (1:200-1:500).
Clonality Monoclonal
Preparation method The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen
Alternative Lysine

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Methylation of lysine residues is a common regulatory posttranslational modification (PTM) that results in the mono-, di-, or tri-methylation of lysine at ε-amine groups by protein lysine methyltransferases (PKMTs). Two PKMT groups are recognized based on structure and catalytic mechanism: class I methyltransferases or seven β strand enzymes, and SET domain-containing class V methyltransferases. Both use the methyl donor S-adenosyl-L-methionine to methylate histone and non-histone proteins. Class I methyltransferases methylate amino acids, DNA, and RNA. Six methyl-lysine-interacting protein families are distinguished based on binding domains: mBT, PHD finger, Tudor, PWWP, WD40 repeat, and chromodomains. Many of these display differential binding preferences based on lysine methylation state. KDM1 subfamily lysine demethylases catalyze demethylation of mono- and di-methyl lysines, while 2-oxoglutarate-dependent JmjC (KDM2-7) subfamily enzymes also modify tri-methyl lysine residues.
Alternative Lysine
Others The antibody detects endogenous Pan Methylated Lysine protein.

Image & description

Fig.1. Western blot analysis of Hela using Pan Methylated Lysine Monoclonal Antibody.

Fig.1. Western blot analysis of Hela using Pan Methylated Lysine Monoclonal Antibody.

Fig.2. Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma using Pan Methylated Lysine Monoclonal Antibody.

Fig.2. Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma using Pan Methylated Lysine Monoclonal Antibody.

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