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p73 Polyclonal Antibody

p73 Polyclonal Antibody

Views(19) Publications(0) Catalog no(ABP53074)
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Specification

Product name p73 Polyclonal Antibody
Immunogen Synthesized peptide derived from human p73 around the non-acetylation site of K321
Host Rabbit
Reactivity Human
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative TP73; P73; Tumor protein p73; p53-like transcription factor; p53-related protein

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background TP73 encodes a member of the p53 family of transcription factors involved in cellular responses to stress and development. It maps to a region on chromosome 1p36 that is frequently deleted in neuroblastoma and other tumors, and thought to contain multiple tumor suppressor genes. The demonstration that this gene is monoallelically expressed (likely from the maternal allele), supports the notion that it is a candidate gene for neuroblastoma. Many transcript variants resulting from alternative splicing and/or use of alternate promoters have been found for this gene, but the biological validity and the full-length nature of some variants have not been determined.
Gene ID 7161
Alternative TP73; P73; Tumor protein p73; p53-like transcription factor; p53-related protein
Others p73 Polyclonal Antibody detects endogenous levels of p73 protein.
Accession O15350
Observed Band(KD) 70

Image & description

Fig.1. Western Blot analysis of Jurkat cells using p73 Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.1. Western Blot analysis of Jurkat cells using p73 Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.2. Immunohistochemical analysis of paraffin-embedded human-lymph-gland, antibody was diluted at 1:100.

Fig.2. Immunohistochemical analysis of paraffin-embedded human-lymph-gland, antibody was diluted at 1:100.

Fig.3. Immunohistochemical analysis of paraffin-embedded human-lymph-gland, antibody was diluted at 1:100.

Fig.3. Immunohistochemical analysis of paraffin-embedded human-lymph-gland, antibody was diluted at 1:100.

Fig.4. Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:100.

Fig.4. Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:100.

Fig.5. Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:100.

Fig.5. Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:100.

Fig.6. Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:100.

Fig.6. Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:100.

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