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p47-phox (phospho Ser345) Polyclonal Antibody

p47-phox (phospho Ser345) Polyclonal Antibody

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Specification

Product name p47-phox (phospho Ser345) Polyclonal Antibody
Immunogen Synthesized peptide derived from human p47-phox around the phosphorylation site of S345
Host Rabbit
Reactivity Human
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:5000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative NCF1; NOXO2; SH3PXD1A; Neutrophil cytosol factor 1; NCF-1; 47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NCF-47K; Neutrophil NADPH oxidase factor 1; Nox organizer 2; Nox-organizing protein 2; SH3

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The protein encoded by NCF1 (neutrophil cytosolic factor 1) is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in NCF1 have been associated with chronic granulomatous disease.
Gene ID 653361
Alternative NCF1; NOXO2; SH3PXD1A; Neutrophil cytosol factor 1; NCF-1; 47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NCF-47K; Neutrophil NADPH oxidase factor 1; Nox organizer 2; Nox-organizing protein 2; SH3
Others Phospho-p47-phox (S345) Polyclonal Antibody detects endogenous levels of p47-phox protein only when phosphorylated at S345.
Accession P14598
Observed Band(KD) 45

Image & description

Fig.1. Western Blot analysis of Jurkat cells using Phospho-p47-phox (S345) Polyclonal Antibody diluted at 1:1000.

Fig.1. Western Blot analysis of Jurkat cells using Phospho-p47-phox (S345) Polyclonal Antibody diluted at 1:1000.

Fig.2. Western blot analysis of 3T3 lysis using Phospho-p47-phox (S345) antibody. Antibody was diluted at 1:1000.

Fig.2. Western blot analysis of 3T3 lysis using Phospho-p47-phox (S345) antibody. Antibody was diluted at 1:1000.

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