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p21 (phospho Thr145) Polyclonal Antibody

p21 (phospho Thr145) Polyclonal Antibody

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Specification

Product name p21 (phospho Thr145) Polyclonal Antibody
Immunogen Synthesized peptide derived from human p21 around the phosphorylation site of T145
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative CDKN1A; CAP20; CDKN1; CIP1; MDA6; PIC1; SDI1; WAF1; Cyclin-dependent kinase inhibitor 1; CDK-interacting protein 1; Melanoma differentiation-associated protein 6; MDA-6; p21

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background CDKN1A encodes a potent cyclin-dependent kinase inhibitor. Cyclin dependent kinase inhibitor 1A binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of CDKN1A is tightly controlled by the tumor suppressor protein p53, through which cyclin dependent kinase inhibitor 1A mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. Cyclin dependent kinase inhibitor 1A can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. Cyclin dependent kinase inhibitor 1A was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack CDKN1A have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found for CDKN1A.
Gene ID 1026
Alternative CDKN1A; CAP20; CDKN1; CIP1; MDA6; PIC1; SDI1; WAF1; Cyclin-dependent kinase inhibitor 1; CDK-interacting protein 1; Melanoma differentiation-associated protein 6; MDA-6; p21
Others Phospho-p21 (T145) Polyclonal Antibody detects endogenous levels of p21 protein only when phosphorylated at T145.
Accession P38936

Image & description

Fig. Western Blot analysis of various cells using Phospho-p21 (T145) Polyclonal Antibody.

Fig. Western Blot analysis of various cells using Phospho-p21 (T145) Polyclonal Antibody.

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