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P-Selectin Polyclonal Antibody

P-Selectin Polyclonal Antibody

Views(77) Publications(0) Catalog no(ABP53324)
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Specification

Product name P-Selectin Polyclonal Antibody
Immunogen Synthesized peptide derived from the N-terminal region of human P-Selectin
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative SELP; GMRP; GRMP; P-selectin; CD62 antigen-like family member P; Granule membrane protein 140; GMP-140; Leukocyte-endothelial cell adhesion molecule 3; LECAM3; Platelet activation dependent granule-external membrane protein; PADGEM; CD62P

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background SELP encodes a 140 kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. P-selectin redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. The membrane protein is a calcium-dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. Alternative splice variants may occur but are not well documented.
Gene ID 6403
Alternative SELP; GMRP; GRMP; P-selectin; CD62 antigen-like family member P; Granule membrane protein 140; GMP-140; Leukocyte-endothelial cell adhesion molecule 3; LECAM3; Platelet activation dependent granule-external membrane protein; PADGEM; CD62P
Others P-Selectin Polyclonal Antibody detects endogenous levels of P-Selectin protein.
Accession P16109
Observed Band(KD) 91

Image & description

Fig.1. Western Blot analysis of NIH-3T3, KB cells using P-Selectin Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.1. Western Blot analysis of NIH-3T3, KB cells using P-Selectin Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.2. Western Blot analysis of 3T3 cells using P-Selectin Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.2. Western Blot analysis of 3T3 cells using P-Selectin Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.3. Immunohistochemical analysis of paraffin-embedded human-tonsils, antibody was diluted at 1:100.

Fig.3. Immunohistochemical analysis of paraffin-embedded human-tonsils, antibody was diluted at 1:100.

Fig.4. Immunohistochemical analysis of paraffin-embedded human-tonsils, antibody was diluted at 1:100.

Fig.4. Immunohistochemical analysis of paraffin-embedded human-tonsils, antibody was diluted at 1:100.

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