| Product name | One-step TUNEL Apoptosis Assay Kit (Green Fluorescence) |
| Applications notes | Abbkine One-step TUNEL Apoptosis Assay Kit (Green Fluorescence) relies on the presence of nicks in the DNA which can be identified by TdT, an enzyme that catalyzes the addition of dUTPs that are labeled with fluorescein. This kit provides all the essential components with an optimized assay protocol, suitable for fluorescence microplate reader, fluorescence microscope, or flow cytometer. Its signal can be easily detected at the popular FITC channel ( Ex/Em = 490nm/520 nm). |
| Kit components | • TdT Enzyme • Equilibration Buffer (5×) •Label Mix Green •Probe Diulen •DAPI (500×) •BSA Working Solution •TritonX-100 (100%) •DNase I (5 U/µL) •10×DNase I Buffer •Proteinase K (20mg/mL) |
| Features & Benefits | • Optimized assay protocol, suitable for fluorescence microplate reader, fluorescence microscope, or flow cytometer. • Signal can be easily detected at the popular FITC channel (Ex/Em = 490nm/520 nm). |
| Usage notes | The reaction buffer contains cacodylate and cobalt chloride, highly toxic chemicals. After contact with skin, wash immediately with plenty of water. In case of accident or if you feel unwell, seek medical advice immediately. Do not drink, eat or smoke when using. |
| Storage instructions | Stable for at least 6 months at -20°C from date of shipment. Once opened, refer to list of materials supplied for storage conditions of individual components. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | One of the most easily measured features of apoptotic cells is the break-up of the genomic DNA by cellular nucleases. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) is a method for detecting DNA fragmentation by labeling the 3′-hydroxyl termini in the double-strand DNA breaks generated during apoptosis. The TUNEL assay relies on the presence of nicks in the DNA which can be identified by TdT, an enzyme that catalyzes the addition of dUTPs that are labeled with fluorescein. |
Fig. Fluorescence images using Abbkine TUNEL Apoptosis Detection Kit (Green Fluorescence) in HeLa cells with the treatment of 10 U/mL Dnase I for 10min.
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