| Product name | NUMB Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the Internal region of human NUMB |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000;IF/ICC1:100-500;ELISA 1:5000-20000 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | NUMB; Protein numb homolog; h-Numb; Protein S171 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 70kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Protein numb homolog encoded by NUMB plays a role in the determination of cell fates during development. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. Alternative splicing results in multiple transcript variants. |
| Gene ID | 8650 |
| Alternative | NUMB; Protein numb homolog; h-Numb; Protein S171 |
| Others | NUMB Polyclonal Antibody detects endogenous levels of NUMB protein. |
| Accession | P49757 |
| Observed Band(KD) | 70 |
Fig. Western Blot analysis of A549 cells using NUMB Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.
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