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Nrf2 Polyclonal Antibody

Nrf2 Polyclonal Antibody

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Specification

Product name Nrf2 Polyclonal Antibody
Immunogen Synthesized peptide derived from the C-terminal region of human Nrf2 at AA range: 530-610
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IF, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IF (1:200-1:1000), IHC-P (1:100-1:300), ELISA (1:40000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative NFE2L2; NRF2; Nuclear factor erythroid 2-related factor 2; NF-E2-related factor 2; NFE2-related factor 2; HEBP1; Nuclear factor; erythroid derived 2, like 2

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 75-100 KD
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background NFE2L2 encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for NFE2L2.
Gene ID 4780
Alternative NFE2L2; NRF2; Nuclear factor erythroid 2-related factor 2; NF-E2-related factor 2; NFE2-related factor 2; HEBP1; Nuclear factor; erythroid derived 2, like 2
Others Nrf2 Polyclonal Antibody detects endogenous levels of Nrf2 protein.
Accession Q16236

Image & description

Fig.1. Western Blot analysis of various cells using Nrf2 Polyclonal Antibody diluted at 1:1000.

Fig.1. Western Blot analysis of various cells using Nrf2 Polyclonal Antibody diluted at 1:1000.

Fig.2. Western Blot analysis of HUVEC cells using Nrf2 Polyclonal Antibody diluted at 1:1000.

Fig.2. Western Blot analysis of HUVEC cells using Nrf2 Polyclonal Antibody diluted at 1:1000.

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