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Nox3 Polyclonal Antibody

Nox3 Polyclonal Antibody

Views(263) Publications(0) Catalog no(ABP51978)
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Specification

Product name Nox3 Polyclonal Antibody
Immunogen Synthesized peptide derived from the Internal region of human Nox3 at AA range: 260-340
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:40000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative NOX3; MOX2; NADPH oxidase 3; Mitogenic oxidase 2; MOX-2; gp91phox homolog 3; GP91-3

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background NOX3 encodes a member of the NOX family of NADPH oxidases. These enzymes have the capacity to generate superoxide and other reactive oxygen species (ROS) and transport electrons across the plasma membrane. The ROS generated by family members have been implicated in numerous biological functions including host defense, posttranlational processing of proteins, cellular signaling, regulation of gene expression, and cell differentiation. NADPH oxidase 3 encoded by NOX3 is expressed predominantly in the inner ear and is involved in the biogenesis of otoconia/otolith, which are crystalline structures of the inner ear involved in the perception of gravity.
Gene ID 50508
Alternative NOX3; MOX2; NADPH oxidase 3; Mitogenic oxidase 2; MOX-2; gp91phox homolog 3; GP91-3
Others Nox3 Polyclonal Antibody detects endogenous levels of Nox3 protein.
Accession Q9HBY0
Observed Band(KD) 65

Image & description

Fig.1. Western Blot analysis of various cells using Nox3 Polyclonal Antibody.

Fig.1. Western Blot analysis of various cells using Nox3 Polyclonal Antibody.

Fig.2. Western blot analysis of various cell lysates, antibody was diluted at 1:1000. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.2. Western blot analysis of various cell lysates, antibody was diluted at 1:1000. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

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