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NOS3 (phospho Thr494) Polyclonal Antibody

NOS3 (phospho Thr494) Polyclonal Antibody

Views(174) Publications(0) Catalog no(ABP55400)
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Specification

Product name NOS3 (phospho Thr494) Polyclonal Antibody
Immunogen Synthesized peptide derived from human NOS3 around the phosphorylation site of T494
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:5000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative NOS3; Nitric oxide synthase; endothelial; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in NOS3 are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for NOS3.
Gene ID 4846
Alternative NOS3; Nitric oxide synthase; endothelial; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII
Others Phospho-NOS3 (T494) Polyclonal Antibody detects endogenous levels of NOS3 protein only when phosphorylated at T494.
Accession P29474
Observed Band(KD) 140

Image & description

Fig. Western Blot analysis of 293 cells using Phospho-NOS3 (T494) Polyclonal Antibody diluted at 1:1000.

Fig. Western Blot analysis of 293 cells using Phospho-NOS3 (T494) Polyclonal Antibody diluted at 1:1000.

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