|Product name||Normal Donkey Serum|
|Applications||BL, ELISA, ICC, IF, IHC|
|Applications notes||Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC-p (1:10-20), IF/ICC (1:10-20) and ELISA (1:100).|
|Preparation method||Normal donkey serum is processed from blood collected from non-immunized normal adult donkeys. Hemolysis is minimized by employing proprietary techniques during the blood collection and serum extraction processes.|
|Preparation method||Lipid extracted and dialyzed against 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2|
|Storage buffer||10 mM sodium phosphate, 0.15 M sodium chloride with 0.05% sodium azide as preservative, pH 7.2|
|Storage instructions||Store at 2-8°C or aliquot and store at or below -20°C for long-term storage. Avoid repeated freezing and thawing.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||Normal donkey serums, which are from non-immuned donkey hosts, are lipid extracted to improve clarity, dialyzed against phosphate buffered saline (PBS) containing sodium azide. Normal serum diluted to 5%-20% (v/v) in PBS is strongly recommended as a blocking reagent to reduce background from non-specific binding, or control for most immunoassay applications. Best results are obtained with diluted normal serum from the same host as the labeled antibody, as a separate incubation step before addition of the primary antibody.|
Fig. Normal donkey serum is processed from blood collected from non-immunized normal adult donkeys. Normal serum diluted to 5%-20% (v/v) in PBS is strongly recommended as a blocking reagent to reduce background from non-specific binding, or control for most immunoassay applications. 2ml, 10ml package sizes are available, whicle bulk size available upon request.
Author：Ma A, Gou M, Song T Publication name：Genomics IF：6.205
Author：Hao J, Li S, Shi X, et al Publication name：Sci Rep, 2018, 8(1): 4516. IF：4.29
Author：Zhang Z, Yu Q, Zhang X, Wang X, Su Y, He W, Li J, Wan H, Jing X Publication name：Life Sci IF：3.647
Author：Fang P, Sun G, Wang J Publication name：FEBS Open Bio IF：2.231
1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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