| Product name | NGF Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the Internal region of human NGF |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC: 1:100-300;ELISA 1:20000;IF 1:100-300;Not yet tested in other applications. |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | NGF; NGFB; Beta-nerve growth factor; Beta-NGF |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 27kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | NGF is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. Nerve growth factorp has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in NGF have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of NGF's expression is associated with allergic rhinitis. |
| Gene ID | 4803 |
| Alternative | NGF; NGFB; Beta-nerve growth factor; Beta-NGF |
| Others | NGF Polyclonal Antibody detects endogenous levels of NGF protein. |
| Accession | P01138 |
| Observed Band(KD) | 27 |
Fig.1. Western Blot analysis of various cells using NGF Polyclonal Antibody diluted at 1:2000.
Fig.2. Western Blot analysis of Jurkat cells using NGF Polyclonal Antibody diluted at 1:2000.
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