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IPKine™ HRP, Goat Anti-Rabbit IgG HCS

IPKine™ HRP, Goat Anti-Rabbit IgG HCS

Views(4226) Publications(16) Catalog no(A25222)
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Product name IPKine™ HRP, Goat Anti-Rabbit IgG HCS
Immunogen Rabbit IgG Fc Fragment
Host Goat
Reactivity Rabbit
Applications IP, WB
Applications notes The suggested dilution ratio is: WB: 1:1,000-1:10,000 (1:2000 optimal), IP: 1:1,000-1:10,000 (1:2000 optimal).
Clonality Polyclonal
Preparation method Affinity purified using solid phase Rabbit IgG(H+L) Fc Fragment with finally > 95% purity based on SDS-PAGE
Conjugate HRP

Product Properties

Formulation Liquid solution
Features & Benefits 50kD or 25kD protein detection on Western blots after Immunoprecipitation is often suffered from heavy or light chain blotting contamination. Abbkine's IPKine™ products solve these problems through less background noise, and enhanced accuracy. Their light chain/Fc fragment specific optimization will eliminate heavy/light chain interference, while serum proteins absorbed minimize cross-reactivity with immunoglobulins from other species which may be present on blots.
Storage buffer Liquid in PBS, pH 7.4, containing 1% BSA as stablizer and 50% Glycerol.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Abbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application.
Others The antibody reacts with Fc portion of rabbit IgG heavy chain, while it doesn’t react with the Fab portion of rabbit IgG. It's cross-reaction with human serum proteins has been specialized minimized, while it may cross-react with immunoglobulins from other species. It has no reactivity on non-immunoglobulin serum proteins

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