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NF-E4 (Acetyl Lys43) Polyclonal Antibody

NF-E4 (Acetyl Lys43) Polyclonal Antibody

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Specification

Product name NF-E4 (Acetyl Lys43) Polyclonal Antibody
Immunogen Synthesized peptide derived from the human NF-E4 around the acetylation site of K43
Host Rabbit
Reactivity Human
Applications ELISA, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:20000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative NFE4; Transcription factor NF-E4

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The erythroid-specific protein encoded by NFE4, and the ubiquitous transcription factor CP2, form the stage selector protein(SSP) complex, which is involved in preferential expression of the gamma-globin genes in fetal erythroid cells. Alternate use of an in-frame upstream non-AUG(CUG) translation initiation codon, and a downstream AUG codon, results in two isoforms. While the long isoform(22 kDa) acts as an activator, the short isoform(14 kDa) has been shown to repress gamma-globin gene expression. NFE4 is located in an intron of the FBXL13 gene on the opposite strand.
Gene ID 58160
Alternative NFE4; Transcription factor NF-E4
Others Acetyl-NF-E4 (K43) Polyclonal AntibodySynthesized peptide derived from the human NF-E4 around the acetylation site of K43.
Accession Q86UQ8
Observed Band(KD) 19

Image & description

Fig.1. Western Blot analysis of AD-293 cells using Acetyl-NF-E4 (K43) Polyclonal Antibody. Secondary Antibody was diluted at 1:20000.

Fig.1. Western Blot analysis of AD-293 cells using Acetyl-NF-E4 (K43) Polyclonal Antibody. Secondary Antibody was diluted at 1:20000.

Fig.2. Western Blot analysis of AD-293 cells using Acetyl-NF-E4 (K43) Polyclonal Antibody. Secondary Antibody  was diluted at 1:20000.

Fig.2. Western Blot analysis of AD-293 cells using Acetyl-NF-E4 (K43) Polyclonal Antibody. Secondary Antibody was diluted at 1:20000.

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