| Product name | NDUFS1 Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the C-terminal region of human NDUFS1 at AA range: 590-670 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:40000;IF 1:50-200 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | NDUFS1; NADH-ubiquinone oxidoreductase 75 kDa subunit; mitochondrial; Complex I-75kD; CI-75kD |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 80kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | The multisubunit NADH: ubiquinone oxidoreductase (75 kDa subunit, mitochondrial) (NDUFS1) is the first enzyme complex in the electron transport chain of mitochondria. It is also named as Complex I-75kD. By use of chaotropic agents, complex I can be fragmented into 3 different fractions: a flavoprotein fraction, an iron-sulfur protein (IP) fraction, and a hydrophobic protein (HP) fraction. NDUFS1 is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. |
| Gene ID | 4719 |
| Alternative | NDUFS1; NADH-ubiquinone oxidoreductase 75 kDa subunit; mitochondrial; Complex I-75kD; CI-75kD |
| Others | NDUFS1 Polyclonal Antibody detects endogenous levels of NDUFS1 protein. |
| Accession | P28331 |
| Observed Band(KD) | 80 |
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