| Product name | NCX1 Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the Internal region of human NCX1 at AA range: 270-350 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;ELISA 1:5000;Not yet tested in other applications. |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | SLC8A1; CNC; NCX1; Sodium/calcium exchanger 1; Na(+)/Ca(2+)-exchange protein 1 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 108kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | In cardiac myocytes, Ca(2+) concentrations alternate between high levels during contraction and low levels during relaxation. The increase in Ca(2+) concentration during contraction is primarily due to release of Ca(2+) from intracellular stores. However, some Ca(2+) also enters the cell through the sarcolemma (plasma membrane). During relaxation, Ca(2+) is sequestered within the intracellular stores. To prevent overloading of intracellular stores, the Ca(2+) that entered across the sarcolemma must be extruded from the cell. The Na(+)-Ca(2+) exchanger is the primary mechanism by which the Ca(2+) is extruded from the cell during relaxation. In the heart, the exchanger may play a key role in digitalis action. The exchanger is the dominant mechanism in returning the cardiac myocyte to its resting state following excitation. |
| Gene ID | 6546 |
| Alternative | SLC8A1; CNC; NCX1; Sodium/calcium exchanger 1; Na(+)/Ca(2+)-exchange protein 1 |
| Others | NCX1 Polyclonal Antibody detects endogenous levels of NCX1 protein. |
| Accession | P32418 |
| Observed Band(KD) | 108 |
Fig. Western Blot analysis of extracts from 293 cells, using NCX1 Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.
You must be logged in to post a review.
Reviews
There are no reviews yet.